Proteinase K Powder 100mg

Availability: In stock

$85.00

Quick Overview

Genomic PCR Grade Proteinase K Powder 


Proteinase K is a stable and highly reactive serine protease


Catalog No. 505-PKP


Stable for 2 years at 2-8℃

Details

Proteinase K is an endoproteolytic enzyme that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. Proteinase K is classified as a nonspecific serine protease. The smallest peptide to be hydrolysed is a tetrapeptide.


Applications: Proteinase K effectively digests native proteins. It can be used to inactivate DNases and RNases when isolating DNA and RNA from tissues or cells.


Usage Conditions:

• Proteinase K is active over a wide pH range—optimal activity between 6.5 and 9.5

• Under denaturing conditions—e.g., in the presence of SDS or urea

• In the presence of metal chelating agents—e.g., EDTA

• At high temperatures—optimum digestion temperature is 65°C


Unit Definition: One unit of the enzyme liberates folin-positive amino acids and peptides corresponding to 1μmol tyrosine in 1min at 37°C using denatured hemoglobin as substrate.


Quality Control: Tested for the absence of endo-, exodeoxyribonucleases and ribonucleases